Introduction of hydroxyl-bearing amino acids causes bathochromic spectral shifts in rhodopsin. Amino acid substitutions responsible for red-green color pigment spectral tuning.
نویسندگان
چکیده
منابع مشابه
Mechanisms of spectral tuning in the mouse green cone pigment.
Diversification of cone pigment spectral sensitivities during evolution is a prerequisite for the development of color vision. Previous studies have identified two naturally occurring mechanisms that produce variation among vertebrate pigments by red-shifting visual pigment absorbance: addition of hydroxyl groups to the putative chromophore binding pocket and binding of chloride to a putative e...
متن کاملTerminal amino acids of rhodopsin.
Rhodopsin, the photosensitive pigment of rod vision, is a chromoprotein composed in equimolar parts of the protein opsin and the carotenoid retinene (1). Its absorption spectrum and molar extinction are known (2) and the kinetics of bleaching by light and of regeneration have been investigated (3). An upper limit of 40,000 has been set for its molecular weight (cattle) (1). Since rhodopsin is i...
متن کاملTerminal Amino Acids of Rhodopsin
Rhodopsin, the photosensitive pigment of rod vision, is a chromoprotein composed in equimolar parts of the protein opsin and the carotenoid retinene (1). Its absorption spectrum and molar extinction are known (2) and the kinetics of bleaching by light and of regeneration have been investigated (3). An upper limit of 40,000 has been set for its molecular weight (cattle) (1). Since rhodopsin is i...
متن کاملMolecular mechanism of long-range synergetic color tuning between multiple amino acid residues in conger rhodopsin
The synergetic effects of multiple rhodopsin mutations on color tuning need to be completely elucidated. Systematic genetic studies and spectroscopy have demonstrated an interesting example of synergetic color tuning between two amino acid residues in conger rhodopsin's ancestral pigment (p501): -a double mutation at one nearby and one distant residue led to a significant λ(max) blue shift of 1...
متن کاملNew primers for the avian SWS1 pigment opsin gene reveal new amino acid configurations in spectral sensitivity tuning sites.
Recently, polymerase chain reaction-based estimates of visual pigment spectral tuning from genomic DNA have offered an alternative to the authoritative but rather slow and complicated retinal microspectrophotometry method. The genomic DNA method involves sequencing a fragment of the short-wavelength sensitive pigment, type 1 (SWS1) opsin gene covering amino acid positions 86, 90, and 93 and has...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)50115-6